The growing threat of drug-resistant bacteria, highlights the importance of developing new antibacterial strategies. Lantibiotics are promising candidates for the development of new antibiotics. Some of the prominent lantibiotics are subtilin, gallidermin, epidermin, duramycin and nisin. However, the best studied and most commonly used lantibiotic is nisin. Nisin modification machinery is of special interest because it is able to modify, export and process any peptide if the peptide is fused to the NisA leader peptide. NisB and NisC are supposed to localize at the cytoplasmic membrane; this makes the interaction with the integral membrane protein NisT possible. It is proposed that these three proteins form a synthetase complex, NisBTC.
Identify subcellular localization of the “Gram-positive specific” lantibiotics, in particular nisin, that target the bacterial cell wall, both in the producer and target bacteria, with the ultimate goal to develop more efficient production systems for these valuable compounds.
Fluorescent microscopy, mass spectrometry, HPLC and FACS.