Subcellular localization of lantibiotic biosynthesis machinery and its interactions
Fig. 2 Interactions of NisB, NisC, NisT and nisin prepeptide
Understanding the maturation of nisin and the interaction of nisin with its modification machinery will provide mechanistic information which might help in the development of novel antibiotics.
The aim of this project is the in vivo characterization of the putative NisBTC complex using single molecule fluorescence microscopy. Detailedly, the goals are:
1. Identify subcellular localization of lantibiotics (in particular nisin) biosynthesis machinery.
2. Investigate interaction of NisB, NisC and NisT.
3. Assess times of expression and assembly of nisin biosynthesis complexes.
Construction of plasmids; Fluorescence microscopy; Time-lapse microscopy; Mass spectrometry; HPLC (High Performance Liquid Chromatography); FACS (Fluorescence Activated Cell Sorter); Western blot.