Title: Back to the roots: Mining for novel antimicrobial compounds from seed endophytes and deciphering the endophytic mechanism
Plant seeds can benefit from seed-associated microorganisms, of which seeds endophytes are of particular interest as they are transmitted from generation to generation. In our current research, we isolated culturable endophytic bacteria from onion seeds and found that the endophytic bacterium Paenibacillus showed antagonistic activity against a wide range of different plant fungal, oomycetal, bacterial pathogens and possessed PGPR properties, such as siderophore production, protease activity, phosphorus solubility etc. Furthermore, Paenibacillus also possessed antagonistic activity against some clinical and food spoilage pathogens, such as Pseudomonas aeruginosa, Bacillus cereus etc. We sequenced the whole genome of the isolated bacteria and conducted a genome mining approach. Different bacteriocins, NRPS, PKS-NRPS hybrids and volatile compounds were discovered. Comparative transcriptomic analysis of the Paenibacillus confronting with different pathogens, together with chemical characterization, in vitro and in vivo plant assays are performed. To sum up, we have isolated promising bacterial sources to discover novel antimicrobial compounds from endophytes and to elucidate the mechanism of plant-microbe interaction, which can be applied in agricultural and/or industrial applications.
To decipher: i) what are the novel antimicrobials produced by the endophytic bacteria against the antagonists; ii) what are the mode of action for the endophytic bacteria while interacting with the host plant. iiii) how do the endophytic bacteria affect the growth and development of the host plant
Genomic analysis (Genome mining etc.)
Transcriptomic analysis/Quantitative PCR
Chemical extractions and elucidation